Ultrasensitive tumour-penetrating nanosensors of protease activity

Ultrasensitive tumour-penetrating nanosensors of protease activity

The ability to identify cancer lesions with endogenous biomarkers is currently limited to tumours ~1 cm in diameter. We recently reported an exogenously administered tumour-penetrating nanosensor that sheds, in response to tumour-specific proteases, peptide fragments that can then be detected in the urine. Here, we report the optimization, informed by a pharmacokinetic mathematical model, of th...

Photoactivated Spatiotemporally-Responsive Nanosensors of in Vivo Protease Activity.

Proteases play diverse and important roles in physiology and disease, including influencing critical processes in development, immune responses, and malignancies. Both the abundance and activity of these enzymes are tightly regulated and highly contextual; thus, in order to elucidate their specific impact on disease progression, better tools are needed to precisely monitor in situ protease acti...

Hybrid in vivo FMT-CT imaging of protease activity in atherosclerosis with customized nanosensors.

OBJECTIVE Proteases are emerging biomarkers of inflammatory diseases. In atherosclerosis, these enzymes are often secreted by inflammatory macrophages, digest the extracellular matrix of the fibrous cap, and destabilize atheromata. Protease function can be monitored with protease activatable imaging probes and quantitated in vivo by fluorescence molecular tomography (FMT). To address 2 major co...

Multiplex detection of protease activity with quantum dot nanosensors prepared by intein-mediated specific bioconjugation.

We report here a protease sensing nanoplatform based on semiconductor nanocrystals or quantum dots (QDs) and bioluminescence resonance energy transfer (QD-BRET) to detect the protease activity in complex biological samples. These nanosensors consist of bioluminescent proteins as the BRET donor, quantum dots as the BRET acceptor, and protease substrates sandwiched between the two as a sensing gr...

Ultrasensitive and Selective Multiplexing Detection of Cancer Markers Using Nanowire Nanosensors